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For the enzymes AAT, GmDH, ME, GPI, LDH and IDH, nine, seven, four, nine, seven, and four different phenotypes, respectively, were observed. All six isolates of an unidentified sterile Pythium sp. isolated from field soil showed the same band positions for all six enzymes compared. These phenotypes were not similar to any of the known Pythium species. Two isolates of unknown Pythium species (145 and 299) showed the same band positions for all six enzymes. The phenotypes for all three unknown Pythium spp. were different from the other species in the experiment. Five isolates of P. heterothallicum showed the same band positions far all enzymes compared except one enzyme, IDH. Two isolates of P. torulosum showed the same band positions for enzymes AAT, GmDH and ME, and three isolates of P. totulosum showed the same positions for enzymes GPI, LDH, and IDH. Single isolates of P. spinosum and P. irregulare showed the same band positions for enzymes AAT, GmDH and GPI. In conclusion, sterile types of Pythium species showed 100% similarities among themselves but did not show any similarity with all isolates of P. heterothallicum and P. spinosum isolate, and showed very low similarities with other isolates in general except with unknown Pythium isolate 306. Similarity levels between different species were low in general with few exceptions.
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